Faculty Research Interests
Xin Jie Chen, PhD
- Professor of Biochemistry and Molecular Biology
Research Programs and Affiliations
- Biochemistry and Molecular Biology
- Biomedical Sciences Program
Education & Fellowships
- PhD: University of Paris-Sud, 1987, Molecular and Cellular Genetics
Mitochondrial biogenesis and inheritance, aging and aging-related degenerative diseases.
Link to PubMed (Opens new window. Close the PubMed window to return to this page.)
Mitochondria are the powerhouses that generate energy by oxidative phosphorylation (OXPHOS) to support cellular activities, and are the integrators of cellular signals that promote different forms of cell death. Mitochondria are also known as the “powerhouses of diseases and aging”. Mitochondrial dysfunction is associated with a rapidly growing number of aging-related neuromuscular degenerative diseases and metabolic disorders. How the mitochondrial function deteriorates during aging and how this in turn induces cellular degeneration are not well understood. We use yeast, cultured human cell lines and mouse as model systems to address the following questions.
(1) We are interested in understanding how mitochondrial dysfunction contributes to aging and aging-related neuromuscular diseases.
(2) We are interested in identifying evolutionarily conserved pathways that can potentially delay and possibly, reverse mitochondria-induced cellular degeneration.
(3) We investigate the mechanisms of mitochondrial DNA recombination, replication and repair.
Wang, X. and Chen, X.J. (2015) A cytosolic network suppressing mitochondria-mediated proteostatic stress and cell death. Nature, Jul 20. doi: 10.1038/nature14859 [Epub ahead of print]
Liu, Y., Wang, X. and Chen, X.J. (2015) Misfolding of mutant adenine nucleotide translocase in yeast supports a novel mechanism of Ant1-induced muscle diseases. Mol. Biol. Cell 26:1985-1994.
Mbantenkhu, M., Wierzbicki, S., Wang, X., Guo, S., Wilkens, S., Chen, X.J. (2013) A short carboxyl-terminal tail is required for single-stranded DNA binding, higher-order structural organization, and stability of the mitochondrial single-stranded annealing protein Mgm101. Mol Biol Cell 24:1507-18.
La T, Clark-Walker GD, Wang X, Wilkens S, Chen XJ. (2013) Mutations on the N-terminal edge of the DELSEED loop in either alpha or beta subunit of the mitochondrial F1-ATPase enhance ATP hydrolysis in the absence of the central gamma rotor. Eukaryote Cell 12:1451-61
Wang X, Mbantenkhu M, Wierzbicki S, Chen XJ (2013) Preparation of the Mgm101 recombination protein by MBP-based tagging strategy. J Vis Exp Jun 25;(76). doi: 10.3791/50448.
Liu Y, Chen XJ. (2013) Adenine nucleotide translocase, mitochondrial stress, and degenerative cell death. Oxid Med Cell Longev. 2013:146860. Epub 2013 Jul 18.
Chen, X.J. (2013) Mechanism of homologous recombination and implications for aging-related deletions in mitochondrial DNA. Microbiology and Molecular Biology Reviews 77:476-96.
Nardozzi, J.D. *, Wang, X.* (* equal contribution), Mbantenkhu, M., Wilkens, S. and Chen, X.J. (2012) A properly configured ring structure is critical for the function of the mitochondrial DNA recombination protein, Mgm101. J Biol Chem 287:37259-68.
Mbantenkhu, M.*, Wang, X.* (* equal contribution), Nardozzi, J.D., Wilkens, S., Hoffman, E., Patel, A., Costrove, M.S. and Chen, X.J. (2011) Mgm101 is a Rad52-related protein required for mitochondrial DNA recombination. J Biol Chem 286:42360-70.
Chen, X.J. (2011) The search for nonconventional mitochondrial determinants of aging. Mol Cell 42:271-273.
Wang X, Salinas K, Zuo X, Kucejova B, Chen XJ. (2008) Dominant membrane uncoupling by mutant adenine nucleotide translocase in mitochondrial diseases. Hum Mol Genet. 17:4036-44.
Wang X, Zuo X, Kucejova B, Chen XJ. Reduced cytosolic protein synthesis suppresses mitochondrial degeneration (2008) Nat Cell Biol. 10:1090-7.
OTHER SIGNIFICANT CONTRIBUTIONS
Chen, X.J. (co-corresponding author), Wang, X.W. and Butow R.A. (2007) Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA. PNAS 104:13738-13743.
Chen, X.J. and Butow, R.A. (2005) Organization and inheritance of mitochondrial nucleoids. Nature Review/Genetics 6:815-825.
Chen, X.J., Wang, X.W., Kaufman, B.A. and Butow, R.A. (2005) Aconitase couples metabolic regulation to mitochondrial DNA maintenance. Science 307:714-717.
Zuo, X.M., Clark-Walker, G.D. and Chen, X.J (2002). The mitochondrial nucleoid protein, Mgm101p, of Saccharomyces cerevisiae is involved in the maintenance of rho+ and ori/rep-devoid petite genomes but is not required for hypersuppressive rho- mtDNA. Genetics 160:1389-1400.
Chen, X.J., Guan, M.X. and Clark-Walker, G,D. (1993) MGM101, a nuclear gene involved in maintenance of the mitochondrial genome in Saccharomyces cerevisiae. Nucleic Acid Res. 21:3473-3477.
Mitochondrial carriers in calcium-signaling and diseases
Kucejova B, Li L, Wang X, Giannattasio S, Chen XJ. (2008) Pleiotropic effects of the yeast Sal1 and Aac2 carriers on mitochondrial function via an activity distinct from adenine nucleotide transport. Mol Genet Genomics. 280:25-39.
Chen, X.J. (2004) Sal1p, a calcium-dependent carrier protein that suppresses an essential cellular function associated with the Aac2p isoform of ADP/ATP translocase in Saccharomyces cerevisiae. Genetics 167:607-617.
Chen, X.J. (2002) Induction of an unregulated channel by mutant nucleotide translocase suggests an explanation for human ophthalmoplegia. Human Molecular Genetics 16: 1835-1843.
Suppression of mitochondrial stress (rho-zero-lethality)
Clark-Walker, G.D. and Chen, X.J. (2001) Dual mutations reveal interactions between components of oxidative phosphorylation in Kluyveromyces lactis. Genetics 159, 929-938.
Chen, X.J. and Clark-Walker, G.D. (2000) The yeast petite mutation: fifty years on. International Review of Cytology. 194, 197-237. (invited review, featured research on the cover page).
Chen, X.J. (corresponding author) and Clark-Walker, G.D. (1999) Alpha and beta subunits of F1-ATPase are required for survival of petite mutants in Saccharomyces cerevisiae. Mol. Gen. Genet. 262, 898-908.
Chen, X.J. and Clark-Walker, G.D. (1996) The mitochondrial genome integrity gene, MGI1, of Kluyveromyces lactis encodes the beta-subunit of F1-ATPase. Genetics 144:1445-1454.
Clark-Walker, G.D. and Chen, X.J. (1996) A vital function for mitochondrial DNA in the petite-negative yeast Kluyveromyces lactis. Mol. Gen. Genet. 252:746-750.
Chen, X.J. and Clark-Walker, G.D. (1995) Specific mutations in alpha- and gamma-subunits of F1-ATPase affect mitochondrial genome integrity in the petite-negative yeast Kluyveromyces lactis. EMBO J. 14:3277-3286.
Chen, X.J. and Clark-Walker, G.D. (1993) Mutations in MGI genes convert Kluyveromyces lactis into a petite-positive yeast. Genetics 133:517-525.