Services: Molecular weight determination and sample requirements

Protein dissolved in 0.1% formic acid with 5% (or higher) acetonitrile is infused into a mass spectrometer, the m/z -mass to charge ratios are measured, and the molecular weight is determined.

Service	Service Code	Sample Requirements
Protein samples are submitted to the facility for molecular mass determination.	200	Protein samples can be submitted either as soluble lyophilized powder or in solution with adequate buffer Total protein in a sample: ≥ 20 picomole/protein Sample buffer: 0.1% formic acid, 5% (or higher) acetonitrile Samples should not contain detergents, salts, etc.