Services: Molecular weight determination and sample requirements

Protein dissolved in 0.1% formic acid with 5% (or higher) acetonitrile is infused into a mass spectrometer, the m/z -mass to charge ratios are measured, and the  molecular weight is determined.

Service Service Code Sample Requirements
Protein samples are submitted to the facility for molecular mass determination.
N-200
  • Protein samples can be submitted either as soluble lyophilized powder or in solution with adequate buffer
  • Total protein in a sample: ≥ 20 picomole/protein
  • Sample buffer: 0.1% formic acid, 5% (or higher) acetonitrile
  • Samples should not contain detergents, salts, etc.