One of many research initiatives in the Matthews' lab focuses on the role of extracellular matrix and cell surface glycoproteins in the developing nervous system and in learning, memory, plasticity and diseases. This slide shows extracellular matrix (ECM) staining on a glioma initiating cell.

Neuroscience Program Faculty

Richard D Veenstra, PhD

Richard D Veenstra, PhD
Appointed 10/01/86
3162 Weiskotten Hall
766 Irving Ave.
Syracuse, NY 13210

315 464-5540

Current Appointments

Hospital Campus

  • Downtown

Research Programs and Affiliations

  • Biomedical Sciences Program
  • Neuroscience Program
  • Pharmacology

Education & Fellowships

  • PhD: University of Iowa, 1983

Research Interests

  • Regulation of connexin-specific-gap-junctions; gap junction channel biophysics.


Link to PubMed External Icon (Opens new window. Close the PubMed window to return to this page.)


The contractile function of the heart is controlled by the electrical activity initiated in the pacemaker sinoatrial node and conducted throughout the heart. An electrical impulse, called an action potential, is generated by specific ion channels that are directly regulated by membrane voltage or ionic blockade. The rapid transmission of this 1/10th of a volt signal is accomplished by gap junctions, a type of intercellular junction which forms a tunnel-like channel. Gap junctions are typically open at rest and, hence, do not require voltage-dependent activation. Little is known about how gap channel proteins, called connexins, conduct electric current and how this current flow is regulated by physiological or pathophysiological (disease) conditions.

By producing site-directed mutations in the two major cardiac gap junction proteins, connexin43 (Cx43) and connexin40 (Cx40), we are examining the molecular basis for the selective electrical conductance and molecular permeability properties of cardiac gap junctions. We hope to make structural inferences about gap junction channel pore structure by observing how endogenous polyamines, small polybasic molecules derived from amino acids such as spermine, block Cx40 and three other connexin gap junctions while having no effect on Cx43 and the majority of other 20 mammalian connexin-specific gap junctions. Intracellular calcium elicits myocardial contraction and, at least under pathophysiological conditions, can shut down gap junction communication. Recently published observations provide new insights as to how this is effected and we are studying how the (patho)physiological regulation of cardiac, lens, and liver gap junctions may be altered by naturally occurring mutations. We are also examining the mechanisms by which human atrial fibrillation mutations in Cx40 or Cx43 alter cardiac gap junction function to produce cardiac arrhythmias or other human diseases.

There is no known clinical therapeutic pharmacology for gap junctions despite their importance to the conduction of the heartbeat for every second of life. We have investigated the mechanism by which the first experimental gap junction agonist, rotigaptide, helps preserve gap junction communication during a heart attack and slow the onset of lethal cardiac arrhythmias. We have also begun investigating what effects novel types of anti-cancer drugs called histone deacetylase (HDAC) inhibitors have on cardiac gap junctions and the action potential they conduct. Preliminary evidence suggests that under-appreciated protein post-translational modifications like acetylation and arginylation modulate the cardiac action potential and its conduction in addition to phosphorylation. These studies involving novel protein post-translational modifications and experimental/clinical drugs are continuing in the laboratory.

Selected Publications

  1. Veenstra RD and RL DeHaan. Measurement of single channel currents from cardiac gap junctions. Science 233: 972-974, 1986.
  2. Veenstra RD, H-Z Wang, DA Beblo, MG Chilton, AL Harris, EC Beyer, and PR Brink. Selectivity of connexin-specific gap junction channels does not correlate with channel conductance. Circ. Res. 77: 1156-1165, 1995.
  3. Lin X, E Fenn, and RD Veenstra. An amino terminal lysine residue of connexin40 that is required for spermine block. J. Physiol. 570: 251-269, 2006.
  4. Lin X, C Zemlin, J Hennan, JS Petersen, and RD Veenstra. Enhancement of ventricular gap junction coupling by rotigaptide. Cardiovasc Res 79: 416-426, 2008.
  5. Lin X, J Gemel, A Glass, CW Zemlin, EC Beyer, and RD Veenstra. Connexin40 and connexin43 determine gating properties of atrial gap junction channels. J Molec Cell Cardiol. 48: 238-245, 2010.
  6. Chen Y, Zhou Y, Lin X, Wong H-C, Xu Q, Jiang J, Wang S, Lurtz MM, Louis CF, Veenstra RD, Yang JJ. Molecular interaction and functional regulation of connexin50 gap junctions by calmodulin. Biochem J. 435: 711-722, 2011.
  7. Xu Q, Chen Y, Kopp R, Yang JJ, Roe MW, Veenstra RD. Calcium regulation of connexin43 via a cytoplasmic loop calmodulin binding domain gating mechanism. Am J Physiol Cell Physiol 302:C1548-C1556, 2012.




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Additional Collaborators

  • Paul Gold, PhD External Icon
    Professor, Biology (Syracuse University)
    Research Interests: Aging, Cell Signaling and Communication, Learning, Memory, and Plasticity, Neurological and Psychiatric Conditions.
  • James Hewett, PhD External Icon
    Associate Professor, Biology (Syracuse University)
    Research Interests: Neuroscience and Central Nervous System Neurobiology and Pathology: Neuromodulators and Epilepsy: Arachidonic Acid Metabolism and Cyclooxygenase-2: Cytokines and Interleukin-1beta: Signal Transduction and Gene Expression.
  • Sandra Hewett, PhD External Icon
    Professor, Neuroscience, Biology (Syracuse University)
    Research Interests: Mechanisms underlying cell death in the central nervous system: the interplay between excitotoxicity and inflammation.
  • Donna Korol, PhD External Icon
    Associate Professor, Biology (Syracuse University)
    Research Interests: Neural mechanisms of learning and memory across the lifespan.
  • Katharine (Kate) Lewis, PhD External Icon
    Associate Professor, Biology (Syracuse University)
    Research Interests: Specification and patterning of spinal cord interneurons; Formation of functional neuronal circuitry; Evolution of spinal cord patterning and function; Dorsal-ventral neural tube patterning; zebrafish development.