ACADEMIC PROFILE
Lisi Krainer

"Being responsible for my own research project has been a great incentive to step out of my scientific comfort zone and explore areas less familiar to me such as Molecular Biology. These bacteria were transformed to produce a plasmid containing a synthetic piece of double stranded DNA I designed." - Lisi Krainer

Medical and Graduate Courses

Faculty

Robert W Zajdel, PhD

Robert W Zajdel, PhD
Appointed 08/29/91
143 Weiskotten Hall
766 Irving Ave.
Syracuse, NY 13210

315 464-8530

Current Appointments

Hospital Campus

  • Downtown

Education & Fellowships

  • PhD: SUNY Health Science Center at Syracuse, 1997

Clinical Interests

  • Clinical anatomy.

Education Interests

  • Teaching in the medical college and health professions.  Current classes and experience include Medical Gross anatomy, Microscopic anatomy, Neuroanatomy, Physical Therapy anatomy, Medical Cellular Physiology- small group, Biochemistry and Cell Biology-Post baccalaureate program.

    Advisory Dean for MS1-4.  MSPE letter writer.

Research Interests

  • Not currently doing wet lab research. Expression of myofibrillar proteins at the early stages of heart development.

Research Abstract

Striated muscle tropomyosin is classically described as consisting of 10 exons, 1a, 2b, 3, 4, 5, 6b, 7, 8, and 9a/b, in both skeletal and cardiac muscle. Experiments in our laboratory have demonstrated expression of a naturally occurring chimeric isoform of tropomyosin that has both smooth and striated muscle exons. The chimeric isoform has a replacement of exon 2a for 2b that is commonly found in striated isoforms. GFP constructs were used to demonstrate translation and incorporation into organized myofibrils. Antisense oligonucleotide transfection decreased the expression of this isoform and disrupted organization and function.

Selected References

Wang J., H. Thurston,  E. Essandoh, M. Otto, M. Han, A. Rajan, S. Dube, R. W. Zajdel, J. M. Sanger,  K.K. Linask, D.K. Dube, J.W. Sanger. (2008) Tropomyosin expression and dynamics in developing avian embryonic muscles.  Cell Motil. Cytoskel. 65:379-392.

Zajdel, R. W., H. Thurston, S. Prayaga, S. Dube, B.J. Poiesz,  D. K. Dube.  (2007). A reduction of tropomyosin limits development of sarcomeric structures in cardiac mutant hearts of the Mexican axolotl. Cardiovasc Toxicol.  7:235-246.

Zajdel, R. W., M. D. McLean, Denz, C.R., Dube S., Thurston H. L., Poiesz, B. J., D. K. Dube.  (2006).  Differential expression of tropomyosin during segmental heart development in Mexican axolotl.  J. Cell Biochem. 99: 952-965.

Zajdel, R. W., C. R. Denz, A. Narshi, Syamalima Dube, D. K. Dube. (2005).  Anti-sense mediated inhibition of expression of the novel striated tropomyosin isoform TPM1κ disrupts myofibril organization in embryonic axolotl hearts.   J. Cell. Biochem.   95: 840-848.

Denz, C. R., A. Narshi, R. W. Zajdel, D. K. Dube. (2004).  Expression of a novel cardiac-specific tropomyosin isoform in humans.  Biochem. Biophys. Res. Commun.  320: 1291-129.

 

 

 

 

Faculty Profile Shortcut: http://www.upstate.edu/faculty/zajdelr